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. 2022 Apr 16;12:6398. doi: 10.1038/s41598-022-10476-6

Figure 3.

Figure 3

NNMT deficiency ameliorates renal fibrotic changes in the UUO model. (A) NNMT mRNA expression and NNMT activity, NAMPT, NMNAT, QPRT, ACMSD, and NAPRT mRNA expression were measured in NNMT-KO mice and WT littermates after UUO induction. The values shown are means ± SEM (n = 4–7 per group). (B) Renal fibrosis was assessed by Masson-trichrome staining in the kidneys of NNMT-KO mice and WT littermates after UUO induction. The values shown are means ± SEM (n = 3–7 per group). (C) Fibrosis-related genes were measured in the kidneys of NNMT-KO mice and WT littermates after UUO induction. The data represent means ± SEM (n = 4–7 per group). (D) Renal function (serum creatinine and urea nitrogen) was measured in NNMT-KO mice and WT littermates after UUO induction. The values shown are means ± SEM (n = 4–7 per group). UUO, unilateral ureter obstruction; NNMT, nicotinamide N-methyltransferase; NMNAT, nicotinamide mononucleotide adenyltransferase; NAMPT, nicotinamide phosphoribosyltransferase; QPRT, quinolinate phosphoribosyltransferase; ACMSD, alpha-amino-beta-carboxy-muconate-semialdehyde decarboxylase; NAPRT, nicotinate phosphoribosyltransferase; Col, collagen; CTGF, connective tissue growth factor; TGF, transforming growth factor. *P < 0.05, **P < 0.01 versus WT mice. †P < 0.05, ††P < 0.01 versus the same genotype control mice (sham-operated kidney).