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. 2022 Apr 16;12:6398. doi: 10.1038/s41598-022-10476-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NNMT deficiency ameliorates renal inflammation in the UUO model. Renal NNMT mRNA expression (A), inflammation-related genes (B), renal NAD + metabolites (D), and mRNA expression of sirtuins (E) were measured in the kidneys of NNMT-KO mice and WT littermates two days after UUO induction. The data represent means ± SEM (n = 4–8 per group). (C) IHC staining and quantitative analysis for F4/80. The data represent means ± SEM (n = 3–8 per group). (F) The acetylation status of NF-κB in the kidney after UUO induction (n = 4 per group). Full-length blots/gels are presented in Supplementary Fig. 7. UUO, unilateral ureter obstruction; NNMT, nicotinamide N-methyltransferase; Il-1β, interleukin 1-beta; MCP-1, monocyte chemotactic protein-1; TNF-α, tumor necrosis factor-alpha; NAM, nicotinamide; NMN, nicotinamide mononucleotide; NAD + , nicotinamide adenine dinucleotide; MNA, 1-methylnicotinamide; N-Me-2PY, N-methyl-2-pyridone-5-carboxamide; N-Me-4PY, N-methyl-4-pyridone-3-carboxamide; NF-κB, nuclear factor-kappa B. *P < 0.05, **P < 0.01 versus WT mice; †P < 0.05, ††P < 0.01 versus the same genotype control mice (sham-operated kidney).