Fig. 3.

PDGFB is mainly secreted by astrocytes after SCI. A–D Immunofluorescence staining of PDGFB (red), GFAP (green) and PDGFRβ (white) in sagittal sections at 14 dpi. E–H Higher magnification images of the boxed region in D. Arrows indicate GFAP⁺PDGFB⁺ cells. Arrowheads indicate PDGFRβ⁺PDGFB⁺ cells. I–L Immunofluorescence staining of PDGFB (red), CD31 (green) and nuclei (blue) in sagittal sections at 14 dpi. J–L represent boxed region in I. M–P Immunofluorescence staining of PDGFB (red), CD68 (green) and nuclei (blue) in sagittal sections at 14 dpi. N–P represent boxed region in M. Q Quantification of the proportion of GFAP⁺PDGFB⁺ cells or PDGFRβ⁺PDGFB⁺ cells in PDGFB⁺ cells at 14 dpi. Asterisks indicate the injured core. Scale bars: 200 μm in D, I and M and 10 μm in H, L and P. n = 5 animals per group