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. 2022 Apr 16;19:95. doi: 10.1186/s12974-022-02449-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Intrathecal injection of SU16f promotes axon regeneration after SCI. A–H Immunofluorescence staining of NF (red) and GFAP (green) in sagittal sections of the Control and SU16f groups at 28 dpi. The region of interest (ROI) represents the boxed region on the left. I–N Immunofluorescence staining of 5-HT (red) and GFAP (green) in sagittal sections of the Control and SU16f groups at 28 dpi. O–Q Higher magnification images of the boxed region in J and M. R Quantification of the density of NF⁺ axons in the GFAP⁻ area at 28 dpi. S Quantification of the percentage of 5-HT⁺ area in the area of the spinal cord segment spanning the injured core at 28 dpi. Asterisk indicates the injured core. Scale bars: 200 μm in G and N, 20 μm in H and 50 μm in Q. *P < 0.05 and ****P < 0.0001 by Student’s t test, n = 5 animals per group