Skip to main content
. Author manuscript; available in PMC: 2023 Apr 7.
Published in final edited form as: Structure. 2022 Feb 10;30(4):510–521.e3. doi: 10.1016/j.str.2022.01.006

Figure 4: Quantification of the spatial distribution of mitochondria-insulin vesicle contacts and localization with respect to PM.

Figure 4:

A) Comparison of the number of mitochondria-insulin vesicle contacts per cell normalized by cytosolic volume. The mean value of contacts at each time shows a significant difference between unstimulated and 5-min stimulated cells with high glucose (*P=0.0126) (left panel), and 1-min stimulated cells with 25mM glucose+Ex-4 (*P=0.0267) (right panel) (Tukey’s multiple comparison test). B) Frequency distribution of mitochondria-vesicle contacts with respect to the PM; the distance is normalized by cell size. C) Average distance from the PM after 25 mM glucose of stimulation (left) and 25 mM glucose+Ex-4 (right). No significance difference was seen in cells treated with glucose alone. A significant difference in the distance was observed after 5-min stimulation with glucose+Ex-4 with respect to 1 min (*P=0.0453) and 30 min (*P=0.0397) (Tukey’s multiple comparison test).