Table 1: Quantitative analysis of the segmented organelles.

List of volume and LAC values of cells, nuclei, mitochondria, cytosol, and insulin vesicles for each condition and time point. The mean numbers of insulin 432 vesicles per condition and percentage of vesicles in contact with mitochondria are also reported. Each value is 433 reported with its standard deviation.

Stimulation	0 min (n=8)	25 mM glucose			25 mM glucose + 10 nM Ex-4			10 nM Ex-4
		1 min (n=4)	5 min (n=8)	30 min (n=8)	1 min (n=4)	5 min (n=8)	30 min (n=8)	5 min (n=4)	30 min (n=4)
Cell volume (|jm3)	715±148	636±137	752±125	873±261	633±138	1224±304	733±246	1040±232	801±157
Nucleus volume (jm3)	199±32	180±31	238±51	228±61	196±27	397±97	203±74	334±38	300±65
Nucleus volume (%)	28±4	31±23	32±5	27±38	29±3	33±2	28±5	33±5	37±4
Nucleus LAC (jm−1)	0.24±0.01	0.27±0.02	0.28±0.02	0.25±0.02	0.29±0.02	0.26±0.02	0.27±0.02	0.26±0.02	0.24±0.02
Mitochondria volume (jm3)	32±11	42±3	43±10	38±12	51±9	73±15	41±7	86±37	60±21
Mitochondria volume (%)	4.4±0.9	6.8±0.9	5.8±1.4	4.5±1.3	8.2±1.3	6.4±0.9	5.9±1.3	8.5±3.4	7.5±2.0
Mitochondria LAC (jm−1)	0.32±0.02	0.35±0.03	0.36±0.02	0.32±0.03	0.36±0.02	0.33±0.02	0.35±0.03	0.32±0.03	0.30±0.03
Cytosol volume (jm3)	516±130	441±110	514±96	644±205	453±113	827±210	530±181	706±197	501±103
Cytosol volume (%)	72±4	69±2	68±5	72±5	71±3	68±2	74±3	67±5	63±4
Cytosol LAC (jm−1)	0.25±0.01	0.28±0.02	0.29±0.02	0.28±0.01	0.28±0.03	0.27±0.03	0.28±0.03	0.26±0.01	0.25±0.02
Insulin vesicle total number	319±93	310±13	409±139	787±204	380±106	386±186	515±156	280±140	244±97
Insulin vesicle total volume (jm3)	4.0±1.9	3.1±1.3	4.0±2.6	7.5±2.3	3.2±0.8	3.8±2.0	5.4±2.8	4.7±3.9	2.1±0.9
Insulin vesicle total volume (%)	0.56±0.28	0.48±0.14	0.55±0.41	0.89±0.30	0.55±0.26	0.31±0.14	0.73±0.32	0.43±0.36	0.29±0.15
Insulin vesicle LAC (jm−1)	0.37±0.03	0.42±0.02	0.46±0.03	0.40±0.02	0.44±0.04	0.45±0.03	0.43±0.03	0.44±0.02	0.43±0.04
Mitochondria-connected nsulin vesicles (%)	9.7±6.1	14.7±6.2	21.1±9.8	10.3±2.9	23.8±15.7	20.1 ±11.7	15.3±5.7	16.9±8.9	28.8±10.1