Key resource table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides and Recombinant proteins
Addex Bio Optimized RPMI media	Addex Bio	Cat# C0004-02
Heat inactivated FBS	Fisher Scientific	Cat# 35-011-CV
PBS	Thermo Scientific	Cat# 10010023
1xPen/Strep	Invitrogen	Cat# 15140-148
β-mercaptoethanol	Invitrogen	Cat# 21985-023
Glucose	Sigma	Cat# 67021
NaCl	Sigma	Cat# S9888
NaHCO3	Sigma	Cat# S5761
KCl	Sigma	Cat# 746436
MgCl2	Sigma	Cat# M2670
CaCl2 × 2H2O	Sigma	Cat# C7902
BSA	Sigma	Cat# A3912
HEPES	Sigma	Cat#H4034
Exendin-4	Med Chem Express	Cat# HY-13443
Critical Commercial Assays
Luminescent ATP Detection Assay Kit	Abcam	Cat# Ab113849
Deposited Data
Raw and analyzed data (no stimulated cells, 5 min and 30 min 25 mM glucose stimulated cells and 25 mM glucose+Ex-4 stimulated cells)	(White et al., 2020)	N/A
Raw and analyzed data (1 min 25 mM glucose stimulated cells and 25 mM glucose+Ex-4 stimulated cells; 5 min and 30 min 10 nM Ex-4 stimulated cells)	This paper	N/A
Experimental Models: Cell Lines
INS-1E	Addex Bio	Cat# C0018009
Software and Algorithms
ImageJ - Fiji 1.53 c	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
GraphPad Prism 9.2	GraphPad	https://www.graphpad.com/scientificsoftware/prism/
Amira 2019.1	Thermo Scientific	https://thermofisher.com/amira-avizo
IMOD - 4.10.42	(Kremer et al., 1996)	https://bio3d.colorado.edu/imod/
AREC reconstruction	(Parkinson et al., 2012)	N/A
Organelle distribution pipeline	(White et al., 2020)	N/A
Contact distribution pipeline	This paper	N/A
Identification of LAC and size of in vesicles associated to mitochondria	This paper	N/A