Table 2.
Regression equation, correlation coefficient, linear range, LOD, and LOQ of 10 components in UHPLC-QQQ-MS.
| Analyte | Regression equation | Correlation Coefficient (R2) | Linear range (µg/mL) | LOD (ng/mL) | LOQ (ng/mL) |
|---|---|---|---|---|---|
| Puerarin | y = 181790.01 x + 29.65 | 0.9995 | 0.00774~2.048 | 1.12 | 3.93 |
| Daidzin | y = 703318.66 x + 481.60 | 0.9992 | 0.00826~1.918 | 0.88 | 3.01 |
| Coptisine | y = 926264.11 x – 1293.07 | 0.9998 | 0.00444~0.894 | 0.75 | 2.32 |
| Epiberberine | y = 2870685.59 x + 965.63 | 0.9996 | 0.00174~0.471 | 0.25 | 0.86 |
| Jatrorrhizine | y = 2719691.61 x – 352.51 | 0.9995 | 0.00323~0.906 | 0.50 | 1.73 |
| Berberine | y = 2845616.29x – 1165.64 | 0.9994 | 0.00175~0.4775 | 0.31 | 1.15 |
| Palmatine | y = 3200432.28 x – 521.16 | 0.9994 | 0.00175~0.487 | 0.28 | 0.90 |
| Coumarin | y = 251481.47 x – 53.44 | 0.9998 | 0.00283~0.734 | 0.43 | 1.44 |
| Daidzein | y = 120736.64 x + 16.89 | 0.9995 | 0.00112~0.3055 | 0.17 | 0.63 |
| Cinnamic acid | y = 6148.94 x – 6.12 | 0.9996 | 0.01633~3.695 | 2.09 | 6.46 |
y: peak area of the components; x: concentration in µg/mL.