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. 2021 Feb 4;29(3-4):127–137. doi: 10.1038/s41434-021-00231-3

Fig. 5. NT-3 gene transfer-induced fiber size increase in Cx32 KO muscle.

Fig. 5

Representative images of SDH-stained tissue sections from gastrocnemius muscle showing neurogenic changes in the untreated (UT) muscle at 6 months post gene delivery (a, b). Slow twitch oxidative (STO, dark), fast twitch oxidative (FTO, intermediate) and fast twitch glycolytic (FTG, light) fibers are seen in the intermediate (a) and superficial (b) zones from muscle. Arrows indicate small angular fibers of either histochemical fiber types, STO and FTO (a) and FTG with STO fiber type groupings marked with asterisks (b). Fiber size increases in all fiber subtypes were seen in both females (c) and males (d). The fiber diameter measurements on gastrocnemius (GAS) and tibialis anterior (TA) muscles showed mean fiber diameters were normalized with NT-3 gene therapy (e, f). Error bars are ±SEM; n = 8 per group, *p = 0.0155 (WT vs. UT), *p = 0.025 (NT-3 vs. UT) in GAS; **p = 0.0089 (WT vs. UT), *p = 0.0370 (NT-3 vs. UT) in TA, One-way ANOVA with Tukey’s multiple comparisons test.