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. 2021 Aug 6;29(3-4):157–170. doi: 10.1038/s41434-021-00282-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 11 — Gating strategy stratified cells into three groups, namely, GFP negative, GFP “dim” and GFP “bright”, based on fluorescence intensity. Optimal transfection conditions using HPAE-EB and CRISPR-C7 plasmid resulted in 11.5% RDEB keratinocytes that were highly fluorescent. Data are representative of three replicates from three independent experiments (n = 3).