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. 2021 Aug 6;29(3-4):157–170. doi: 10.1038/s41434-021-00282-6

Fig. 2. DNA encapsulation efficiency assessment with the HPAE-EB polymer and CRISPR-C7 plasmid complexes.

Fig. 2

Experiments were performed at polymer:DNA w/w ratios from 0.25:1 to 30:1 using 1 μg of DNA. At low polymer:DNA ratios of 0.25:1 and 0.5:1, the HPAE-EB polymer is incapable of adequately encapsulating the DNA from the PicoGreen® reagent. However, upon reaching w/w ratios of 5:1 and greater, an encapsulation efficiency of ~70% is achieved and maintained, indicating sufficient binding to protect the DNA. Samples were performed in triplicate and presented as average ± standard deviations (SD) for three independent experiments (n = 3).