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. 2021 Aug 6;29(3-4):157–170. doi: 10.1038/s41434-021-00282-6

Fig. 4. Transfection capability and cytotoxicity of HPAE-EB polymer complexed to CRISPR-C7 plasmid in HEK293 cells.

Fig. 4

a GFP within the CRISPR-C7 plasmid enabled visual transfection in cells. (I) Untreated cells were used as the negative control. (II) Lipofectamine 3000 was used as the commercial reagent for comparison, and treatments with different polymer:DNA w/w ratios and amounts of DNA (III–VIII) were performed. Scale bar 100 µm. Representative images from six replicates of three independent experiments (n = 3). b Cell viability 72 h post transfection in HEK293 cells by alamarBlue™, where preservation of cell metabolic health post transfections using HPAE-EB polymer and Lipofectamine 3000 was observed. Data were collected from six replicates of three independent experiments and presented as means ± SD (n = 3). *p < 0.05, as compared to control values.