Fig. 7. CRISPR–Cas9 gene editing strategy for targeted exonal excision of mutation-containing exons in COL7A1 gene.

a CRISPR-C7 plasmid schematic detailing construct design containing dual-guide RNA sequences and Cas9 coupled with GFP reporter for transfection efficiency evaluation. b Dual-guide RNA strategy for targeted genomic deletion of pathogenic mutation-containing exon 80 in COL7A1 gene.