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. 2021 Aug 6;29(3-4):157–170. doi: 10.1038/s41434-021-00282-6

Fig. 8. PCR analysis of genomic DNA from HEK293 cells transfected with CRISPR-C7 plasmid under different conditions.

Fig. 8

HEK293 cells were treated with a 0.5 µg and b 1 µg of CRISPR-C7 plasmid. Upper arrow at 320 bp represents unedited DNA, while lower arrow at 263 bp represents DNA lacking exon 80. Lipofectamine 3000 was used as the commercial reagent control. Excision efficiency (%) estimated by densitometry analysis showed a maximum editing of 20.3%, achieved using a HPAE-EB:pDNA w/w ratio of 30:1. Data are representative of three independent experiments (n = 3).