Fig. 1.

A - Allelic discrimination plot showing different genotypes of TLR 7 Gene SNP (rs3853839). An allelic discrimination plot or “cluster plot” shows three clusters. The points in each cluster are grouped closely together and express one genotype. At the lower right corner of the plot the cluster represents allele C (homozygote), labelled with VIC® dye. At the Upper left corner of the plot the cluster represents allele G (homozygote), labelled with FAM™ dye. Approximately midway between the allele C and allele G clusters represent both (allele C and allele G—heterozygote).

B - Amplification plot of TLR7 gene expression displaying fluorescence versus cycle number. Amplification plots are created when the fluorescent signal from each sample is plotted against cycle number; therefore, amplification plots represent the accumulation of product over the duration of the real-time PCR experiment.