Table 2.

Reduction in the percentage of viability of blastocystosis cysts in parasite control group (negative control), after exposure to metronidazole (positive control), and after exposure to the three nanocomposites.

	Time of exposure (h)
	1 h	2 h	3 h	4 h	5 h	24 h
Parasite control	0.67 ± 0.58	0.67 ± 1.15	0.33 ± 0.58	0.67 ± 0.58	0.67 ± 1.15	1.67 ± 0.58
Metronidazole control	81.67 ± 2.52	87.67 ± 1.53	91.33 ± 1.53	96.33 ± 1.15	98.67 ± 0.58	99.33 ± 0.58
ZIF-8/KIT-6/Resvetrol
100 µg/ml
Mean ± Sd	50.33 ± 3.06	56.67 ± 2.52	61.67 ± 3.06	70.33 ± 2.08	84.67 ± 2.31	91.33 ± 4.16
P value	0.0004*	0.0002*	0.0005*	0.0004*	0.0003*	0.0003*
200 µg/ml
Mean ± Sd	57.33 ± 5.51	63.67 ± 4.04	72.33 ± 4.16	80.33 ± 3.06	92.33 ± 4.04	95.67 ± 4.16
P value	0.001*	0.001*	0.0007*	0.0003*	0.0003*	0.004*
500 µg/ml
Mean ± Sd	76.33 ± 4.04	84.33 ± 6.11	91.33 ± 5.68	96.67 ± 2.08	99.33 ± 0.58	99.67 ± 0.58
P value	0.0004*	0.0006*	0.0008*	0.0001*	0.04*	0.05*
800 µg/ml
Mean ± Sd	90.67 ± 2.08	94.33 ± 4.73	97.33 ± 2.89	99.33 ± 0.58	99.67 ± 0.58	99.67 ± 0.58
P value	0.008*	0.0003*	0.0001*	0.21	0.42	0.12
ZIF-8/MCM-41/Curcumin
100 µg/ml
Mean ± Sd	39.67 ± 2.3	43.34 ± 3.05	48.67 ± 6.03	53.34 ± 3.21	57.34 ± 2.08	74.67 ± 3.51
P value	0.0004*	0.0002*	0.0005*	0.0004*	0.0003*	0.0003*
200 µg/ml
Mean ± Sd	44.34 ± 2.08	50.34 ± 2.52	54.34 ± 3.06	75.34 ± 2.08	84.34 ± 3.51	90.67 ± 4.73
P value	0.001*	0.001*	0.0007*	0.0003*	0.0003*	0.001*
500 µg/ml
Mean ± Sd	68.34 ± 3.79	73.34 ± 3.06	78.34 ± 2.52	86.67 ± 2.52	91.33 ± 2.08	95.34 ± 2.08
P value	0.0004*	0.0006*	0.0008*	0.0001*	0.04	0.0001*
800 µg/ml
Mean ± Sd	85.00 ± 2.65	87.67 ± 2.08	90.34 ± 2.08	93.33 ± 2.89	96.67 ± 0.58	99.67 ± 0.58
P value	0.008*	0.0003*	0.0001*	0.21	0.42	0.0001*
ZIF-8/KIT-6
100 µg/ml
Mean ± Sd	36.33 ± 0.58	40.33 ± 1.15	44.67 ± 1.15	49.33 ± 0.58	56.67 ± 0.58	63.33 ± 1.15
P value	0.0003*	0.0006*	0.0008*	0.001*	0.005*	0.035
200 µg/ml
Mean ± Sd	41.67 ± 1.53	45.67 ± 1.53	51.33 ± 0.58	56.33 ± 0.58	62.67 ± 0.58	75.67 ± 1.53
P value	0.003*	0.002*	0.003*	0.0045	0.034	0.135
500 µg/ml
Mean ± Sd	49.33 ± 0.58	54.67 ± 1.15	58.67 ± 1.53	64.67 ± 1.53	78.33 ± 2.08	85.33 ± 0.58
P value	0.005*	0.021*	0.053*	0.047*	0.033*	0.211
800 µg/ml
Mean ± Sd	72.67 ± 1.15	76.33 ± 3.06	77.67 ± 2.52	81.67 ± 2.52	86.67 ± 2.08	91.33 ± 0.58
P value	0.008*	0.100	0.021	0.072	0.15	0.40

Data are presented as the mean ± standard deviation (SD), *P < 0.05 is statistically significant, compared to the parasite control.