FIGURE 3.

Multiple label immunofluorescence of mGlu3 labeling in neurons in layer II/III of rat PL mPFC. Tissue was labeled with antibodies recognizing mGlu3 (red), the dendritic marker Map2 (green), as well as the nuclear Hoechst stain (blue). (A,B) In layer II/III, mGlu3 expression (red) is seen diffusely across the neuropil, with labeling within the perisomatic regions of putative pyramidal neurons as well as the apical dendrites. One representative neuron is outlined by the yellow dashed box in (A), and two representative neurons are outlined in (B). (Ai–iv,Bi–iv) Correspond to the boxed areas in (A,B). In both (Aii,iii,Bii,iii) mGlu3 labeling clearly outlines the apical dendrite, as demarcated by Map2 (green) and surrounds the nucleus (blue). Scale bars: 10 μm.