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. 2022 Apr 4;16:849937. doi: 10.3389/fnana.2022.849937

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Copyright © 2022 Woo, Datta and Arnsten.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiple label immunofluorescence of mGlu3 labeling in microglia in layer II/III of rat PL mPFC. Tissue was labeled with antibodies recognizing the microglial protein Iba1 (cyan), mGlu3 (red), the dendritic marker Map2 (green), as well as the nuclear Hoechst stain (blue). (A) There are diffuse Iba1 positive microglial processes across the neuropil in layer III. (Ai–iv) Magnified area of the yellow box in (A), showing an Iba1 positive microglial process (cyan) co-localized with mGlu3 (red), indicated by white arrows. Scale bars: 10 μm (A), 2.5 μm (Ai–iv).