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SSHTN mice showed elevated osteoclastogenesis induced by LPS in vivo. (A) Histological sections of calvariae were obtained from normal control or SSHTN mice after 5 days of daily supracalvarial injection with PBS or LPS. TRAP staining was performed to identify osteoclasts. (B) Numbers of multinucleated TRAP-positive cells in the sagittal suture mesenchyme of calvariae were quantified. Scale bar = 50 μm (n = 4).
