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. 2022 Apr 4;10:864977. doi: 10.3389/fbioe.2022.864977

FIGURE 2.

FIGURE 2

Promoter engineering and screening using droplet-based microfluidic platform. (A) Schematic diagrams of two plasmid libraries pSET152-p SACE_2101 –1035 (lib)-egfp (ATG) and pSET152-p ermE* -spacer (lib)-egfp (ATG). “NNN” indicated the varied regions. (B) Work flow of promoter library construction and droplet microfluidic based high-throughput screening.