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. 2022 Apr 4;10:864977. doi: 10.3389/fbioe.2022.864977

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Copyright © 2022 Yun, Zhang, Li, Wang, Tu, Liu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Promoter screening by FADS. (A) Fluorescence microscopy of droplets containing wild-type stains (p _{SACE_2101} WT, p _ermE* WT) and variants (p _{SACE_2101} library, p _ermE* library) at different time intervals. B: Bright field. F: Fluorescence. Scale bar: 50 μm. (B) Relative strengths of the screened promoter variants in libraries SACE_2101 (lib) (p _{SACE_2101} library) and ermE*(lib) (p _ermE* library) compared to their corresponding WTs (Surrounded by yellow frames). Error bars represented the standard deviation of three biological samples. *p ≤ 0.05 and ***p ≤ 0.001 (Student’s two-tailed t-test).