Skip to main content
. 2022 Apr 4;10:864977. doi: 10.3389/fbioe.2022.864977

FIGURE 5.

FIGURE 5

Improving erythromycin production in S. erythraea NRRL 23338 by promoter engineering and gene overexpression. (A) Constructs of single-gene overexpression driven by p SACE_2101_s32 (a), and p ermE*_s32 (b), and combinational overexpression of SACE_0718 and SACE_0719 driven by p SACE_2101_s32 and p ermE*_s32 , respectively (c). ery genes: SACE_0716, SACE_0717, SACE_0718, SACE_0719, SACE_0720, and SACE_0731. (B) 24-well-plate fermentation results of S. erythraea NRRL 23338 (WT), S. erythraea/pSET152-hyg (152), overexpressing strains and high-producing strain S0. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, and N.S. indicated no significant difference (Student’s two-tailed t-test).