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. 2022 Apr 4;13:823975. doi: 10.3389/fphar.2022.823975

FIGURE 1.

FIGURE 1

The protein levels of NOX1 and NOX4 were increased by DOX both in vivo and in vitro. (A) Representative western blot analysis of NOX1 and NOX4 in myocardial tissue. (B,C) Quantification of NOX1 and NOX4 expression relative to the β-actin level (n = 6 per group). (D,E) Representative images of NOX1 and NOX4 expression in myocardial tissue detected by the immunofluorescence. The nuclei were stained with DAPI (Scale bar = 50 µm). (F) Representative western blot analysis of NOX1 and NOX4 of NRCMs treated with DOX of different concentrations for 24 h (G,H) Quantification of NOX1 and NOX4 expression relative to the β-actin level (n = 3). (I) Representative western blot analysis of NOX 1 and NOX 4 of NRCMs treated with DOX for different time. (J,K) Quantification of NOX1 and NOX4 expression relative to the β-actin level (n = 4–5). *p < 0.05, **p < 0.01, ***p < 0.001, n. s., not significant. NOX, NADPH oxidase; DOX, doxorubicin; cTnI, Cardiac troponin I.