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. 2000 Oct;44(10):2719–2727. doi: 10.1128/aac.44.10.2719-2727.2000

TABLE 1.

Characteristics of trovafloxacin-selected mutants of M. hominis

Strain Amino acid change at the indicated position in the QRDR ofa:
MIC (μg/ml)b of:
GyrA
ParC
ParE
TVA SPX CIP OFX NOR PEF
153 157 189 91 92 95 446 466
PG21 (reference strain) Ser Glu Ala Ser Ser Glu Leu Glu 0.06 0.06 2 1 32 4
First-step mutants
 IT1 —c — — — — — — Lys 0.25 0.12 8 2 128 4
 IT2 — — — — — — — Lys 0.25 0.12 8 2 128 4
 IT3 — — — — — — — Lys 0.25 0.12 8 2 128 4
Second-step mutants
 IIT2A — — Val — — — — Lys 0.5 0.12 8 1 128 4
 IIT2B Leu — — — — — — Lys 2 1 32 2 128 4
 IIT2C Leu — — — — — — Lys 2 1 32 2 128 4
 IIT2D Leu — — — — — — Lys 2 1 32 2 128 4
 IIT2E Leu — — — — — — Lys 2 1 32 2 128 4
 IIT2F Leu — — — — — — Lys 2 1 32 2 128 4
 IIT3A — — Glu — — — — Lys 1 0.5 16 2 128 4
 IIT3B — — Glu — — — — Lys 1 0.5 16 2 128 4
 IIT3C — Lys — — — — — Lys 1 1 16 2 128 4
 IIT3D — — Glu — — — — Lys 1 0.5 16 2 128 4
 IIT3E — — Glu — — — — Lys 1 0.5 16 2 128 4
 IIT3F — — Glu — — — — Lys 1 0.5 16 2 128 4
Third-step mutants
 IIIT2D1 Leu — — — Pro — — Lys 16 64 >128 64 >128 32
 IIIT2D2 Leu — — Ile — — — Lys 16 32 128 64 >128 32
 IIIT2D3 Leu — — — — Gln — Lys 8 8 32 16 >128 16
 IIIT2F1 Leu — — — — — Phe Lys 16 32 128 64 >128 32
 IIIT2F2 Leu — — Ile — — — Lys 16 64 128 64 >128 32
 IIIT2F3 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F4 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F5 Leu — — — — — Phe Lys 16 64 128 64 >128 32
 IIIT2F6 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F7 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F8 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F9 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F10 Leu — — Ile — — — Lys 16 64 >128 64 >128 32
 IIIT2F11 Leu — — Ile — — — Lys 16 64 128 64 >128 32
a

GyrA, ParC, and ParE residue positions are based on the respective gene sequences for M. hominis (4; this study). GyrA positions 153, 157, and 189 correspond to E. coli coordinates 83, 87, and 119, respectively. ParC positions 91, 92, and 95 correspond to E. coli coordinates 80, 81, and 84, respectively. ParE positions 446 and 466 correspond to E. coli coordinates 440 and 460, respectively. 

b

TVA, trovafloxacin; SPX, sparfloxacin; CIP, ciprofloxacin; OFX, ofloxacin; NOR, norfloxacin; PEF, pefloxacin. 

c

—, identical to that in the reference strain, PG21.