Figure 5.

HPLC profiling of B₆ vitamers in leaves and seeds of rice accessions contrasting in total vitamin B₆ contents. (A) Representative HPLC chromatograms of leaf and seed tissues. To facilitate visualization, chromatograms were offset from the baseline of the standards by 300 units for leaves 30 μl injected no glucosidase, 1,200 units for leaves 10 μl injected glucosidase treated, 2,000 units for unpolished seed, and 2,700 units for polished seed. HPLC analysis of B₆ vitamers in leaves (B), unpolished seeds (C), and polished seeds (D). Samples were obtained from plant material grown under greenhouse conditions in experiment 2. Leaves were sampled from 40-day-old plants. Seed samples were obtained from fully mature pannicles and dried before extraction. Data are the mean ± standard deviation of n = 3 biological replicates. The effect of accession on total vitamin B₆ contents in panels (B–D) was determined by one-way ANOVA (α = 0.05) with multiple comparisons and Tukey’s post-hoc test. Statistically significant differences between accessions are denoted by different letters.