Figure 6.

THIC expression in leaves and correlation of accessions with contrasting vitamin B₁ contents. (A) RNA was isolated from plant material used in Figure 4B for qRT-PCR assay of THIC expression. Expression was normalized to UBQ5 and data are the mean ± standard deviation of n = 3 biological replicates. The effect of accession on gene expression was determined by one-way ANOVA (α = 0.05) with multiple comparisons and Tukey’s post-hoc test. Statistically significant differences between accessions are denoted by different letters. Accessions are ordered according to leaf total vitamin B₁ content as determined in Figure 4B. (B–D) Correlation of THIC gene expression from panel (A) with leaf (B) total vitamin B₁ contents in Figure 4B by Pearson’s correlation coefficient test (p < 0.05), unpolished seed from Figure 4C (C), and polished seed total vitamin B₁ contents from Figure 4D (D).