Figure 2. Efficient deletion of Brd4 by Prrx1-Cre recombinase.

Limb buds were collected from CON (Brd4^{f l/wt} and Brd4^{f l/f l}: Prrx1-Cre negative) and cKO (Brd4^{f l/f l}: Prrx1-Cre positive) 14.5 dpc embryos. Quantitative PCR analysis was conducted on DNA isolated from the limb buds. Both male and female embryos were included in the analysis. Deletion of exon three was assessed using two distinct primer pairs targeting the floxed region within exon 3 and evaluated in relation to non-targeted exon six (n = 7) (A). Western blotting for Brd4 in limb bud tissues of 14.5 dpc embryos (B) and quantification when normalized to Gapdh (n = 7 and 5) (C). Boxplots indicate the median, interquartile range, and the minimum and maximum value in each dataset. Individual mice are represented by a single point on the graph. P-values shown on the graphs represent the results of an unpaired, two-tailed t-test between CON and cKO.