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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Bone. 2021 Oct 23;154:116234. doi: 10.1016/j.bone.2021.116234

Figure 6. Depletion of Brd4 prevents early transcription factor expression in ATDC5 cells.

Figure 6.

ATDC5 cells were transfected with non-targeting siRNA (siCtl) or Brd4-targeting siRNA (siBrd4) and cultured in three-dimensional micromass cultures in chondrogenic differentiation media. Schematic of siRNA transfection experiment and data collection points (A). Western blot analysis performed on protein lysates collected two days after transfection (B). RT-qPCR analysis on mRNA isolated two days post-transfection (C). MTS assay conducted two days after transfection (D). RT-qPCR analysis of cell cycle genes (E) and transcription factors performed on mRNA isolated two days post-transfection. Boxplots indicate the median, interquartile range, and the minimum and maximum value in each dataset (n = 3). Individual biological replicates are represented by a single point on the graph. P-values indicate results from unpaired, two-tailed Student’s t-test between siCtl and siBrd4 groups.