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. 2022 Apr 4;10:852244. doi: 10.3389/fcell.2022.852244

FIGURE 2.

FIGURE 2

Effects of HSP27 knockdown on cell activity and autophagy under UVB irradiation. (A,D) HSP27, p-HSP27, p62 and LC3B protein expressions were detected by using Western blotting in HEKs after HSP27 gene knockdown, regarding β-actin as a reference. (B,F–H) HSP27, p62 and LC3B mRNA levels were detected by using RT-qPCR in HEKs after HSP27 gene knockdown, quantified by using ACTB as a reference. (C) The proliferation ability of HEKs was detected by the CCK-8 method at 0, 24, 48 and 72 h after UVB irradiation in the control group and the si-HSP27 group. (E) The LC3B-Ⅱ/Ⅰ protein expressions of HEKs after HSP27 gene knockdown. (I,L) HSP27, p-HSP27, p62 and LC3B protein expressions were detected by using Western blotting in HDFs after HSP27 gene knockdown, regarding β-actin as a reference. (J,N–P) HSP27, p62 and LC3B mRNA levels were detected by using RT-qPCR in HDFs after HSP27 gene knockdown, quantified by using ACTB as a reference. (K) The proliferation ability of HDFs was detected by the CCK-8 method at 0, 24, 48 and 72 h after UVB irradiation in the control group and the si-HSP27 group. (M) The LC3B-II/I protein expressions of HDFs after HSP27 gene knockdown. All the results were expressed as mean ± SD, and the experiment was repeated at least three times (*p < 0.05, **p < 0.01, ***p < 0.005).