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. 2022 Apr 4;10:852244. doi: 10.3389/fcell.2022.852244

FIGURE 3.

FIGURE 3

Effect of HSP27 knockdown on the antioxidant capacity of cells irradiated with UVB. (A,B) Alterations of ROS level in HEKs after HSP27 gene knockdown. (G,H) Alterations of ROS level in HDFs after HSP27 gene knockdown. (C) CAT, SOD1 and SOD2 protein expressions were detected by using Western blotting in HEKs after HSP27 gene knockdown, regarding β-actin as a reference. (I) CAT, SOD1 and SOD2 protein expressions were detected by using Western blotting in HDFs after HSP27 gene knockdown, regarding β-actin as a reference. (D–F) CAT, SOD1, SOD2 mRNA levels were detected by using RT-qPCR in HEKs after HSP27 gene knockdown, quantified by using ACTB as a reference. (J–L) CAT, SOD1, SOD2 mRNA levels were detected by using RT-qPCR in HDFs after HSP27 gene knockdown, quantified by using ACTB as a reference. All the results were expressed as mean ± SD, and the experiment was repeated for at least three times (*p < 0.05, **p < 0.01, ***p < 0.005).