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. 2022 Apr 4;10:852244. doi: 10.3389/fcell.2022.852244

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Copyright © 2022 Wang, Li, Huang, Bai, Jiang, Li, Liu, Wen, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of HSP27 knockdown on skin autophagy and antioxidant capacity of mice irradiated with UVB. (A) p62, LC3B, CAT, SOD1 and SOD2 protein expressions were detected by using Western blotting after HSP27 gene knockdown, regarding GAPDH as a reference. (B) The LC3B-II/I protein expressions after HSP27 gene knockdown. (C–G) p62, LC3B, CAT, SOD1 and SOD2 mRNA levels were detected by using RT-qPCR after HSP27 gene knockdown, quantified by using GAPDH as a reference. All the results were expressed as mean ± SD, and the experiment was repeated at least three times (*p < 0.05, **p < 0.01, ***p < 0.005).