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. 2022 Apr 4;14:727805. doi: 10.3389/fnagi.2022.727805

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Copyright © 2022 Zhang, Lin, Yang, Zou, Xue, Feng and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene annotation and enrichment of target mRNAs in ceRNA networks. (A,B) The enrichment analysis of target mRNAs derived from mm9_circ_004229/mm9_circ_008009/mm9_circ_009789-associated-ceRNA networks [mm9_circ_004229-mRNA (red), mm9_circ_008009-mRNA (blue) and mm9_circ_009789-mRNA (green)]. (A) Enrichment network constructed by enriched terms. (B) Enriched GO term: “Vascular endothelial growth factor receptor signaling pathway” (GO:0048010). The term related mRNAs are as shown in the figure. P < 0.05 is considered as statistically significant. (C,D) The analysis of mm9_circ_003736-associated-ceRNA network. (C) Real-time qPCR to analysis the expression of mm9_circ_003736 in young and aging mice under control and anesthesia/surgery conditions. ****p < 0.0001. All data are presented as mean ± SEM (n = 6). (D) The enrichment analysis of target mRNAs derived from mm9_circ_003736-associated-ceRNA networks under control and anesthesia/surgery conditions. Heatmap representing the differences between control and anesthesia/surgery conditions, colored by p-values, as shown in the legend above. The darker the color, the more statistically significant the term is. Gray indicates no enrichment in the corresponding term, P > 0.05.