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. 2022 Apr 18;20:177. doi: 10.1186/s12967-022-03369-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Cell proliferation, migration and invasion in vitro after silencing each of the selective gene signatures in breast cancer cells. a, b MTT assays in SQSTM1-, GDF9-, LINC01125-, PTGS2-, GVINP1-, and TMEM64-silenced MCF-7 cells. c, d Colony formation assays in SQSTM1-, GDF9-, LINC01125-, PTGS2-, GVINP1-, and TMEM64-silenced MCF-7 cells. e, f Transwell assays in SQSTM1, GDF9-, LINC01125-, PTGS2-, GVINP1-, and TMEM64-silenced MCF-7 cells. g, h Wound healing assays in SQSTM1-, GDF9-, LINC01125-, PTGS2-, GVINP1-, and TMEM64-silenced MCF-7 cells. All representative pictures are shown on the left, and statistical results are shown on the right. Bar graph data are presented as the mean ± SEM; * refers to P < 0.05