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. 2022 Jan 19;145(2):481–489. doi: 10.1093/brain/awab328

Figure 3.

Figure 3

Distinct reactive states in VCP and SOD1 mutant astrocytes. (A) Representative images and quantification of the percentage of cells immunofluorescent for GFAP (green). Mann-Whitney test. One experimental repeat of two lines per group. (B) Heat maps of pan reactive, A1 and A2 markers in control, SOD1 and VCP astrocytes. Gene expression data represent SD from mean of the variance stabilized values across control, SOD1 and VCP astrocytes. Points represent significantly increased transcripts as determined with the Wald test and following correction for multiple testing using the Benjamini and Hochberg method (adjusted P-values ≤ 0.01). (C) Representative images and quantification of the percentage of cells immunofluorescent for C3 (red). Mann-Whitney test. One experimental repeat of two lines per group. (D) Immunofluorescence demonstrating GFAP-positive astrocytes with C3 immunolabelling in wild-type (WT) and SOD1 mutant mice, top graph shows the C3-immunolabelled proportion of GFAP-positive astrocytes. Bottom graphs display the percentage of GFAP-positive cells. Mann-Whitney test. Three mice per group. Data-points for all the immunolabelling quantification are the number of technical repeats (fields) used in the experiment. Data are presented as mean ± SEM. Scale bar = 20 μm (in A and C).