Figure 1.

Celsr2 expression is enriched in mouse and human spinal motor neurons. Using Celsr2^LacZ transgenic mice, Celsr2 expression is detected by anti-β-gal immunostaining. (A–E) E12.5 spinal sections were immunostained for Isl1 (red), β-gal (green) and counterstained for DAPI (blue). The merged image showed that all Isl1-positive cells in the ventral horn (VH) co-labelled by β-gal immunoreactivity (E). (F–J) In adult spinal sections, all ChAT-positive (red) spinal motor neurons co-expressed β-gal (green) indicated by arrows (J). (K–O) In WPC8 human spinal sections, ISL1 and CELSR2 double immunofluorescent staining showed that CELSR2 was expressed in ISL1-positive neurons in the ventral horn (VH) indicated by arrows (O). B–E, G–J and L–O correspond to boxed areas in A, F and K, respectively. Nuclei were counterstained by DAPI (blue).