Figure 2.

POT1(L259S) binds DNA with reduced affinity. (A) SDS-PAGE gel of WT and mutant POT1N. (B) FP assays of WT and mutant POT1N. The data show that POT1(L259S) binds telomeric DNA with ∼3.5-fold reduced affinity compared with the WT protein. mP, milipolarization. (C) Structure of POT1N (green), DNA (blue/orange; PDB ID: 1XJV). (D) Zoom in at the second OB fold of POT1N and where the mutation L259S is located; same residue and nucleotide numbering as in Lei et al. (2004). Residues (L248, F191) involved in contacts with L259S are shown. Also, K247, which makes a weak hydrogen bond with the backbone of the telomeric DNA, is shown. Source data are available for this figure: SourceData F2.