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. 2022 Apr 14;219(6):e20212553. doi: 10.1084/jem.20212553

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© 2022 Castleman et al.

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Figure 1. — Identification of B_ND cells in human PBMCs. (A) Gating strategy used to identify B_ND cells by flow cytometry as lymphocyte single cell live CD3⁻ CD19⁺ CD27⁻ IgD⁺ CD24^−/lo CD38^−/lo CD10⁻ IgM^−/lo, shown here from mild SARS-CoV-2 infection. (B) Frequency of B_ND cells as a percent of the Naive/Transitional (CD27⁻IgD⁺) population or as a percent of B cells (CD19⁺) in healthy controls (HC; n = 10), those immunized (Immuniz.) against SARS-CoV-2 (n = 15), mild (n = 11), or severe SARS-CoV-2 infection (n = 14). (C) Frequency of B_ND cells from individuals with severe SARS-CoV-2 infection (n = 14) as a percent of the Naive/Transitional (CD27⁻IgD⁺) population or as a percent of the B cell (CD19⁺) population and compared to the frequency of Transitional T1, T2, or Naive B cells. Statistics: one-way ANOVA. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001.