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. 2022 Apr 14;219(6):e20212553. doi: 10.1084/jem.20212553

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© 2022 Castleman et al.

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Figure S4. — B_ND cells have reduced BCR signaling in healthy controls and Naive/T2 B cells from healthy controls and severe SARS-CoV-2 infection have comparable levels of BCR signaling. (A) Expression (geometric mean fluorescent intensity) of pSYK Y348, pBLNK Y84, and pPLCγ2 Y759 in Transitional T1, T2, Naive, or B_ND cells from healthy controls (n = 10) after 5-min incubation with anti-IgG (H + L) F(ab’)2. (B) Fold difference between expression level of pSYK, pBLNK, and pPLCγ2 from unstimulated B_ND cells and stimulation with anti-IgG (H + L) F(ab’)2 for 5 min in each patient population from healthy controls (n = 10), those immunized against SARS-CoV-2 (n = 15) or those with mild (n = 10) or severe SARS-CoV-2 infection (n = 12). (C and D) Expression (geometric mean fluorescent intensity) of pSYK Y348, pBLNK Y84, and pPLCγ2 Y759 in Naive B cells (C) or T2 cells (D) from healthy controls (n = 10) and severe SARS-CoV-2 infection (n = 10) after 5-min incubation with anti-IgG (H + L) F(ab’)2. Statistics: one-way ANOVA or unpaired t test; #, P = 0.06; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.