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Optogenetic pyroptosis induction in single cells. Time-lapse fluorescence confocal microscopy of HaCaT cells stably expressing optoCaspase-1 (red). Blue squares mark the regions which are transiently stimulated with the blue light at indicated timepoints. Pyroptotic cells rapidly gain DRAQ7 signal (blue) and are extruded from the monolayer by neighbors. Scale bar, 20 µm.
