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Pyroptotic cell permeabilization in zebrafish. Time-lapse fluorescence confocal microscopy showing a zebrafish keratinocyte expressing opto-zfCaspa (red) and stimulated with blue light. Left, the brightfield channel. Pyroptotic cell is rapidly extruded from the monolayer, which is followed by the rapid membrane permeabilization, loss of cellular content and gain of DRAQ7 signal (turquoise). Scale bar, 20 µm.
