| Antibodies |
|
|
|
| Guinea pig anti-vGlut2 antiserum |
Millipore |
Cat# AB2251 -I; RRID: AB_2665454 |
| Rabbit anti-PSD95 antiserum |
Invitrogen/ThermoFisher |
Cat# 51–6900; RRID: AB_2533914 |
| Rabbit anti-Ibal antiserum |
Wako Chemical |
Cat# 019–19741; RRID: AB_839504 |
| Rat anti-MerTK antiserum |
eBioscience |
Cat# 14–5751-82; RRID: AB_2688282 |
| Rabbit anti-P2RY12 antiserum |
AnaSpec |
Cat# 55043A; RRID: AB_2298886 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Clozapine-N-oxide (CNO) |
National Institute of Mental Health |
CAS# 34233–69-7, MH# C-929 |
| MerTK small-molecule inhibitor, UNC2025 |
Selleck Chemicals |
Cat# S7576 |
| CNO-containing, sustained-release pellets (s.c.) |
Innovative Research of America |
Cat# X-999 |
| Placebo-containing, sustained-release pellets (s.c.) |
Innovative Research of America |
Cat# C-111 |
|
| Critical commercial assays |
|
|
|
| ACTH ELISA kit |
Phoenix Pharmaceuticals |
Cat# EK-001 –21 |
| CORT ELISA kit |
Cayman Chemicals |
Cat# 501320 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: CRH-IRES-Cre+/+ |
Jackson Laboratory |
JAX: 012704 |
| Mouse: tdTomato+/+ Ai14 |
Jackson Laboratory |
JAX: 007914 |
| Mouse: CX3CR1-GFP+/+ |
Jackson Laboratory |
JAX: 005582 |
| Mouse: CX3CR1-BAC-Cre+ [Tg(Cx3cr1-cre)MW126Gsat] |
GENSAT BAC Transgenic Project (Rockefeller University) |
MMRRC: 036395-UCD; MGI: 5311737 |
| Mouse: Gq-DREADD+/− (stop-floxed) |
Jackson Laboratory |
JAX:026220 |
| Mouse: Mertkfl/fl
|
Dr. Carla Rothlin (Yale University); Fourgeaud et al., 2016 |
N/A |
| Mouse: C57BL/6J (wild-type) |
Jackson Laboratory |
JAX: 000664 |
|
| Software and algorithms |
|
|
|
| FIJI |
https://imagej.net/software/fiji/; Schindelin et al., 2012 |
N/A |
| Imaris 3D reconstruction software |
Bitplane |
N/A |
| Strathclyde Electrophysiology Software (Electrophysiology Data Recorder [WinEDR] and Whole-Cell analysis Program [WinWCP]) |
Courtesy of Dr J. Dempster, University of Strathclyde |
N/A |
| Slidebook microscopy software |
3i (Intelligent Imaging Innovations) |
N/A |
| Python-based microglial process dynamics analysis algorithm |
This paper, available: https://github.com/jaclynrbeck/BaramLabMicrogliaTracking
|
N/A |
| ProMoIJ plug-in for FIJI |
Paris et al., 2017 |
N/A |
| MultipleKymograph plug-in for FIJI |
http://www.embl.de/eamnet/html/body_kymograph.html |
N/A |
|
| Other |
|
|
|
| Fine-gauge plastic-coated aluminum mesh platform (mesh dimensions 0.4 X 0.9 cm) |
McNichols Co. |
Cat# 57398 |
