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. 2022 May;28(5):711–728. doi: 10.1261/rna.079083.121

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© 2022 Łabędzka-Dmoch et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — The homozygous ΔCapet127 strain and functional mutants are viable and do not show respiratory deficiency. Growth on solid agar media containing glucose (YPD) or glycerol (YPG) as the carbon source. A series of 10× dilutions from overnight YPD starter cultures of wild-type (BWP17), heterozygous CaPET127/ΔCapet127 (Δpet127HZ), homozygous ΔCapet127 (ΔCapet127), and two independent point mutant Capet127_D375A/Capet127_D375A (D375A) strains were spotted on plates and incubated for 48 h at 30°C or 37°C.