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Purified T. marneffei Pet127 is a processive 5′-to-3′ exoribonuclease. RNA degradation activity of Tm-Pet127 wt and point mutants against. (A) 5′ fluorescein (FAM) labeled single-stranded RNA substrate (ssRNA), (B) 5′ G-rich ssRNA compared to a random sequence, (C) hairpin RNA structure within the mRNA ATP6/8 transcript from C. albicans. Degradation products were analyzed by 20% denaturing TBE-urea PAGE.
