FIGURE 5.

Activating transcription factor 3 (ATF3)/CCL2 regulates levels of apoptosis in rat brain following middle cerebral artery occlusion (MCAO). (a) Immunofluorescence detection of ATF3 and CCL2 localization in neurons. (b) Neuronal damage in rat brain tissues by hematoxylin‐eosin (HE) staining. (c) Neuronal damage in rat brain tissues by Nissl staining. (d) Immunohistochemical detection of microtubule‐associated protein 2 (MAP2) protein expression in rats. (e) Apoptosis in rat neurons by TUNEL assay. (f) PARP and caspase‐3 protein expression in rat brain tissues measured by western blot. Data are expressed as mean ± SD, and statistical significance was determined using one‐way analysis of variance (ANOVA) (d and e) or two‐way ANOVA (f), followed by Tukey multiple comparison test. *#&p < .05