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. 2022 Mar 8;12(4):e2522. doi: 10.1002/brb3.2522

TABLE 1.

Primer sequence used for reverse transcriptase quantitative polymerase chain reaction (RT‐qPCR)

Gene name Primer
TNF‐α Forward: 5′‐CATCTTCTCAAAATTCGAGTGACAA‐3′
Reverse: 5′‐TGGGAGTAGACAAGGTACAACCC‐3′
IL‐1β Forward: 5′‐CAACCAACAAGTGATATTCTCCATG‐3′
Reverse: 5′‐GATCCACACTCTCCAGCTGCA‐3′
IL‐6 Forward: 5′‐GAGGATACCACTCCCAACAGACC‐3′
Reverse: 5′‐AAGTGCATCATCGTTGTTCATACA‐3′
TGF‐β1 Forward: 5′‐CAACCCAGGTCCTTCCTAAA‐3′
Reverse: 5′‐GGAGAGCCCTGGATACCAAC‐3′
IL‐4 Forward: 5′‐GTCATCCTGCTCTTCTTTCTCG‐3′
Reverse: 5′‐TCTGTGGTGTTCTTCGTTGCT‐3′
IL‐10 Forward: 5′‐TGGCCTTGTAGACACCTTGG‐3′
Reverse: 5′‐AGCTGAAGACCCTCAGGATG‐3′
GAPDH Forward: 5′‐CCGTATTCAGCATTCTATGCTCT‐3′
Reverse: 5′‐CTATGAGACGAGGCTGGCAC‐3′

Abbreviations: IL, interleukin; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; TNF‐α, tumor necrosis factor alpha; TGF‐β1, transforming growth factor β1.