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. 2022 Apr 13;9(2):ENEURO.0026-22.2022. doi: 10.1523/ENEURO.0026-22.2022

Figure 1.

Figure 1.

Comprehensive quantification of lung innervation patterns by specific sensory nerve subsets. A, Four approaches used to label specific sensory populations with the fluorescent reporters GFP or tdTomato. B, Histogram of the diameters of conducting airways and blood vessels analyzed. Dotted lines denote 175 and 375 μm. C, Relative quantification of the diameters of conducting airways and blood vessels clustered into “small,” “medium,” and “large” groups. D, Overall quantification of innervation by specific sensory nerve subsets: % of conducting airways with fibers (black), with fibers that project out to the alveolar region (red), and blood vessels with fibers (green). E, % of conducting airways with fibers of specific afferent subsets, clustered by airway diameter. F, Percentage of conducting airways with fibers of specific afferent subsets that project out to the alveolar region, clustered by airway diameter. G, Distance projected from the conducting airway into the alveolar region by fibers of specific afferent subsets (red bars denote median with interquartile range). H, Percentage of blood vessels with fibers of specific afferent subsets, clustered by airway diameter. Data are derived from Pirt-Ai9 [n = 8 mice, 219 conducting airways (CA), 270 blood vessels (BV)], Pirt-AAV-GFPVagal and Pirt-AAV-tdTDRG (n = 3 mice, 229 CA, 131 BV), Pirt-AAV-tdTLung (n = 3 mice, 127 CA, 134 BV), 5HT3-Ai9 (n = 7 mice, 294 CA, 211 BV), Tac1-Ai9 mice (n = 11 mice, 281 CA, 259 BV), Tac1-AAV-GFPVagal (n = 5 mice, 208 CA, 188 BV), Tac1-AAV-tdTDRG (n = 3 mice, 153 CA, 118 BV), TRPV1-AAV-GFPVagal (n = 10 mice, 313 CA, 288 BV), TRPV1-AAV-tdTLung (n = 4 mice, 161 CA, 139 BV), TRPV1-AAV-tdTDRG (n = 3 mice, 82 CA, 57 BV), and CGRP (n = 6 mice, 296 CA, 279 BV).