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. 2022 Apr 14;17:1725–1739. doi: 10.2147/IJN.S345553

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© 2022 Mandras et al.

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Anti-S. pyogenes activity of cNDs and free chitosan on S. pyogenes. S. pyogenes (10⁴ CFUs/mL) were incubated alone or with OSS (10% v/v), free MW or LW chitosan (0.139% m/v) solution, MW or LW cOFND suspensions (10% v/v), and MW or LW cOLND suspensions (10% v/v) in sterile conditions at 37°C and their growth was monitored for 4, 6, and 24 h. At each incubation time, the samples were spread on TSA agar medium to determine the CFUs/mL. Results are shown as means ± SEM from three independent experiments and expressed as Log CFUs/mL. Data were evaluated for significance by ANOVA. vs controls: ^a p<0.0001; ^b p<0.001.