Table 1.

Physico-Chemical Characterization of ND Formulations

Formulation	Average Diameter ± SD (nm)	Polydispersity Index ± SD	Zeta Potential ± SD (mV)	Osmolarity ± SD (mOsm)	Viscosity ± SD (cP)
LW cOFNDs	305.3 ± 18.5	0.20 ± 0.02	31.95 ± 3.05	285 ± 0.4	1.34 ± 0.02
MW cOFNDs	360.8 ± 25.3	0.21 ± 0.01	30.45 ± 2.46	284 ± 0.3	1.39 ± 0.02
LW cOLNDs	420.4 ± 20.7	0.22 ± 0.02	32.46 ± 2.98	282 ± 0.6	1.32 ± 0.01
MW cOLNDs	545.7 ± 32.1	0.19 ± 0.01	30.20 ± 2.86	284 ± 0.5	1.38 ± 0.02

Notes: MW or LW cNDs either loaded with oxygen (cOLNDs) or unloaded (cOFNDs) were in vitro characterized measuring their average diameter, polydispersity index and zeta potential by dynamic light scattering. Osmolarity and viscosity were determined using an osmometer and an Ubbelohde capillary viscosimeter, respectively. Results are shown as mean ± SD from ten ND formulations.