KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies/Tetramer/Beads | ||
| PE/Cy7 anti-mouse CD69 | Biolegend | Cat# 104512; RRID: AB_493564 |
| BV510 anti-mouse CD62L | Biolegend | Cat# 104441; RRID: AB_2561537 |
| FITC anti-mouse/human CD44 | Biolegend | Cat# 103005; RRID: AB_312956 |
| BV421 anti-mouse CD103 | Biolegend | Cat# 121422; RRID: AB_2562901 |
| APC/Cy7 anti-rat CD90/mouse CD90.1 (Thy-1.1) | Biolegend | Cat# 202519; RRID: AB_2201418 |
| PE/Dazzle 594 anti-mouse CD8a | Biolegend | Cat# 100762; RRID: AB_2564027 |
| APC anti-mouse CD45.1 | eBioscience | Cat# 17-0453-82; RRID: AB_469398 |
| PE anti-mouse/human CD44 | Biolegend | Cat# 103008; RRID: AB_312959 |
| BV711 anti-mouse CD186 (CXCR6) | Biolegend | Cat# 151111; RRID: AB_2721558 |
| APC anti-mouse CD183 (CXCR3) | Biolegend | Cat# 126512; RRID: AB_1088993 |
| FITC anti-mose CD127 (Il7ra) | eBioscience | Cat# 11-1271-82; RRID: AB_465195 |
| BV421 anti-mouse CD169 (Siglec-1) | Biolegend | Cat# 142421; RRID: AB_2734202 |
| BV421 anti-mouse/human CD45R/B220 | Biolegend | Cat# 151111; RRID: AB_2721558 |
| AF488 anti-mouse CD103 Antibody | Biolegend | Cat# 121408; RRID: AB_535950 |
| AF647 anti-rat CD90/mouse CD90.1 (Thy-1.1) | Biolegend | Cat# 202508; RRID: AB_492884 |
| PE TRP-2 tetramer(iTAg H-2Kb TRP-2 Tetramer-SVYDFFVWL-P) | MBL International | Cat# TB-5004-1 |
| Anti-PE beads | MACS Miltenyi | Cat# 130-048-801 |
| Purified anti-mouse CD8a | Biolegend | Cat# 100702; RRID: AB_312741 |
| Depleting anti-CD4 (GK1.5) | Bioxcell | Cat# BE0003-1; RRID: AB_1107636 |
| Depleting anti-CD8 (2.43) | Bioxcell | Cat# BE0061; RRID: AB_1125541 |
| Human TruStain FcX | Biolegend | Cat# 422301 |
| BV711 anti-human CD45 | Biolegend | Cat# 304050; RRID: AB_2563466 |
| PE-Dazzle 594 anti-human CD8 | Biolegend | Cat# 344744; RRID: AB_2566515 |
| DAPI | Biolgend | Cat# 422801 |
| anti-human CD8 | Leica Biosystem | Cat# NCL-CD8 (4b11); RRID: AB_563637 |
| anti-human CD69 | Abcam | Cat# ab233396 |
| AF555 anti-human CD8 | Biolgend | Cat# 344702; RRID: AB_1877104 |
| BV421 anti-human CD69 | Biolgend | Cat# 310929; RRID: AB_2561909 |
| Primary anti-human CD103 | ThermoFisher | Cat# 14-1038-82; RRID: AB_467412 |
| AF488 anti-mouse Ig-g | Jackson Immuno Research | Cat# 115-547-003 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Collagenase Type IV | Worthington Biochemical | Cat# CLSS-4 |
| Collagenase Type II | Sigma | Cat# C6885 |
| Dnase I | Sigma | Cat# 10104159001 |
| BSA | Sigma-Aldrich | Cat# 12659-100GM |
| Liberase | Roche | Cat# 5401020001 |
| Percoll | GE | Cat# 17089101 |
| D-Luciferin, Potassium Salt | GoldBio | Cat# LUCK-3G |
| RPMI 1640 | Corning | Cat# 10-040-CV |
| HBSS | VWR | Cat# VWRL0121-0500 |
| Live Dead Kit | ThrermoFisher | Cat# L34962 |
| Bond Dewax Solution | Leica Biosystem | Cat# AR9222 |
| Bond Epitope Retrieval | Leica Biosystem | Cat# AR9590 |
| Tissue Tek Glas | Sakura | Cat# 6419 |
| Critical Commercial Assays | ||
| AF555 Antibody Labeling Kit | ThermoFisher | Cat# A20187 |
| ChromPlex Kit | Leica Biosystem | Cat# DS9665 |
| Vina Green HRP Chromogen Kit | Biocare | Cat# BRR807 |
| Anti-PE microbeads | MACS Miltenyi | Cat# 130-048-801 |
| Anti-CD8a microbeads | MACS Miltenyi | Cat# 130-117-044 |
| RNA extraction Qiagen RNeasy plus micro kit | Quiagen | Cat# 74034 |
| SMART-Seq v4 Ultra Low Input RNA Kit | ClonTech | Cat# 638490 |
| Nextera DNA flex Library prep kit | Illumina | Cat# 20018704 |
| 10× Single cell 3’ V2 chemistry | 10× Genomics | Cat# PN-120237 |
| Deposited Data | ||
| Bulk Rna-Seq (Fig. 1) | This Paper | GEO GSE169237 |
| scRNA-Seq pmel (Fig. 3) | This Paper | GEO GSE171161 |
| scRNA-Seq endogenous (Fig. 5) | This Paper | GEO GSE169238 |
| scRNA-seq human (Fig. 7) | This Paper | dbGap PHS002309.V1.P1 |
| Experimental Models: Cell Lines | ||
| B16-F10 | Isaiah Fidler, MD Anderson Cancer Center | N/a |
| B16-25K | This paper | N/a |
| B16-Luciferase | Dr. Yina Huang, Dartmouth College | N/a |
| Experimental Models: Organisms/Strains | ||
| C57BL/6NCrl | Charles River Laboratories | Cat# CRL27, RRID: IMSR_CRL:27 |
| B6 CD45.1 | Charles River Laboratories | Cat# CRL:564, RRID: IMSR_CRL:564 |
| B6 Thy1.1 | The Jackson Laboratory | Cat# JAX:000406, RRID: IMSR_JAX:000406 |
| B6 Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J | The Jackson Laboratory | Cat# JAX:005023, RRID: IMSR_JAX:005023 |
| C.129S4(B6)-Ifngtm3.1Lky/J | The Jackson Laboratory | Cat# JAX:017580, RRID: IMSR_JAX:017580 |
| Software and Algorithms | ||
| FlowJo v10 | Treestar Inc | RRID: SCR_008520 |
| Prism 8 | Graphpad Inc | RRID: SCR_002798 |
| Seurat v3.0 | https://satijalab.org/seurat/get_started.html | RRID: SCR_016341 |
| Cell Ranger | 10X Genomics | RRID: SCR_017344 |
| Morpheus | Broad Institute | RRID: SCR_017386 |
| RSEM | Bo Li and Colin Dewey | RRID:SCR_013027 |
| ImageJ | Schneider et al. 2012 | RRID: SCR_018407 |
| RStudio | N/A | https://www.rstudio.com/ |
| Monocle 2.0 | Trapnell Lab | http://cole-trapnell-lab.github.io/monocle-release/ |
| velocyto.R (RNA Velocity) | Kharchenlo Lab | https://github.com/velocyto-team/velocyto.R |