Figure 2.

Enzyme‐linked immunosorbent assay (ELISA) and enzyme‐linked immunospot (ELISPOT) assay performed with isolated splenocytes from immunized mice. (A) IL‐2 and IFN‐γ secreted by splenocytes upon stimulation with the S protein were detected by ELISA. (B) IL‐2‐producing splenocytes after S protein stimulation. (C) IFN‐γ‐producing splenocytes after S protein stimulation. Representative images of spots around the mean value for the groups are also shown in the left panels of (B) and (C). N = 6, points represent individual mice. Data were compared using one‐way analysis of variance followed by Dunnett's multiple comparisons tests, with Group c as the control. *p < 0.05; **p < 0.01. PBS, phosphate‐buffered saline